Sanger Sequencing Single Pass

Introduction to Sanger Sequencing

The Sanger sequencing test is a widely utilized method for DNA sequencing, offering precise results for researchers and clinicians. This test has become a cornerstone in genetic analysis, allowing for the identification and study of genetic variations.

How the Sanger Sequencing Test Works

The Sanger sequencing test involves the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication. The process results in the generation of DNA fragments of various lengths, which are then separated by electrophoresis to determine the DNA sequence.

Applications of Sanger Sequencing

This test is instrumental in various fields, including genetics, forensics, and molecular biology. It is commonly used for identifying mutations, diagnosing genetic disorders, and conducting evolutionary studies.